Deteksi Gen GST (Glutathione S-Transferase ) pada Nyamuk Aedes aegypti Resistensi Insektisida Metomil Metode Real-Time

Authors

  • Anita Dwi Anggraini Poltekkes Kemenkes Surabaya, Indonesia

DOI:

https://doi.org/10.59141/jsi.v7i2.302

Keywords:

Nyamuk Aedes aegypti, Insektisida Metomil, Gen GST, Real-Time PCR

Abstract

Latar Belakang: Salah satu masalah kesehatan utama masyarakat Indonesia adalah DBD, penyakit ini terus meningkat setiap tahun. Sehingga, perlu dilakukan pencegahan dengan cara pengendalian vektor yaitu pemberian insektisida yang dilanjut dengan pemeriksaan berbasis molekuler. Penelitian ini bertujuan untuk mendeteksi adanya gen GSTpada nyamuk Aedes aegypti yang resisten terhadap insektisida metomil. Metode: Penelitian ini menggunakan deskriptif kuantitatif untuk mengetahui adanya gen (Glutathioone S-Transferase) dan pengumpulan data dilakukan secara observasi atau pengambilan secara langsung. Hasil: Pada uji resistensi didapatkan 54,66% yaitu 41 ekor dari 75 ekor nyamuk yang resisten terhadap paparan insektisida metomil. Nyamuk yang resisten dilanjutkan untuk deteksi gen GST dengan hasil yang muncul berupa nilai CT. Hasil yang didapatkan dari 4 sampel uji mununjukkan hasil N/A pada nilai CT. Kesimpulan: Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan bahwa 100% sampel uji negatif atau tidak terdeteksi gen GST.

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Published

2025-03-25

How to Cite

Anggraini, A. D. (2025). Deteksi Gen GST (Glutathione S-Transferase ) pada Nyamuk Aedes aegypti Resistensi Insektisida Metomil Metode Real-Time. Jurnal Sehat Indonesia (JUSINDO), 7(2), 629–636. https://doi.org/10.59141/jsi.v7i2.302

Issue

Vol. 7 No. 2 (2025): Jurnal Sehat Indonesia (JUSINDO)

Section

Articles

License

Copyright (c) 2025 Anita Dwi Anggraini

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